| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dinh, Nhien Y (7)
|
PVNCAP |
28.85 |
24.19 |
-4.66 |
| 2 |
Zabst, Helena R (7)
|
PVNCAP |
28.49 |
28.56 |
0.07 |
| 3 |
Ahuja, Rayna L (7)
|
PVNCAP |
|
28.60 |
- |
| 4 |
Yarmosh, Scarlet J (7)
|
PVMACH |
29.72 |
30.17 |
0.45 |
| 5 |
Ghazal, Jordana C (7)
|
PVMACH |
34.21 |
30.51 |
-3.70 |
| 6 |
Lawyer, Lillie M (7)
|
PVNCAP |
29.51 |
31.72 |
2.21 |
|
Pierouchakos, Alexandra G (7)
|
PVBWST |
|
DQ |
- |
|
Reiley, Claire M (7)
|
PVMACH |
|
DQ |
- |
|
Nguyen, Phoebe K (7)
|
PVNCAP |
|
DQ |
- |
|
Weidner, Maya A (7)
|
PVNCAP |
|
DQ |
- |
|
Ismail, Saara F (7)
|
PVMACH |
|
NS |
- |
|
Alexandrescu, Katherine M (7)
|
PVNCAP |
36.59 |
NS |
- |